CeCyD estimates the duration of each cell cycle phase for any kind of cell subjected to BrdU/EdU
incorporation assays. In order to do so, CeCyD uses the inputs provided by the user to feed the
Willians [1] and Stanner & Till [2] equations, as proposed by
da Silva et al. [3].
CeCyD is a free software licensed under the terms of GNU-

Methodology

This page provides a quick overview of the methodology applied. For a more in depth description, please check
da Silva article.
$$\begin{equation}
x = \frac{\ln {(1-\frac{y}{2})}}{-\alpha}
\end{equation}$$
Where $x$ is the duration of the stage in question, $y$ is the cumulative $\%$ cells from G1 to the stage in question,
$\alpha = \frac{\ln{2}}{T}$ (where $T$ is the generation time)
$$\begin{equation}
S = \frac{1}{\alpha} \ln{[L + e^{\alpha Z}]} - (Z+t)
\end{equation}$$